FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F05_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F05_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248927
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA146120.44974848619251834No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT107610.3312170448889741No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG96260.2962824341698044No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG83280.256330782439864No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG74820.2302914162121833No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG69500.21391677929359448No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTCCTTTGGCATATCC63180.19446420310459422No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA56290.17325720153145946No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA56230.17307252517523478No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT55300.17021004165375214No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC53890.165870147282472No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA53490.16463897157430746No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG52330.16106856202063022No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA51990.16002206266869032No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT50830.15645165311501305No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG50650.155897624046339No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG49780.15321981688108105No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA49650.15281968477592756No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT49440.15217331752914115No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA46830.14413989603336733No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT46270.14241625004193692No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT45940.14140053008270115No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG45800.14096961858484355No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC44750.13773778235091155No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA44470.13687595935519634No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG44250.13619881271570583No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG41960.12915033178646365No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG41440.1275498033658497No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA41170.1267187597628386No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAACAA41060.12638018644309337No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT39600.12188639510829267No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG39390.12124002786150628No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA38890.11970105822630057No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT37400.11511492871338752No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT36920.11363751786359004No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG36300.11172919551593495No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA36150.11126750462537324No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT34580.10643513997082728No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG34300.10557331697511207No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT33370.10271083345362945No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC33070.10178745167250602No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC32890.10123342260383197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)