FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F05_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F05_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693293
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC65840.3888281590959155No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC59860.35351235728252584No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG56330.332665404038167No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA50590.2987669588192947No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC43200.25512418701311584No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC40310.23805685135413657No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA39560.23362761199627No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA38160.22535969852825236No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT37070.2189225373281529No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC36590.21608782413911828No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC36220.21390273272257074No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT35330.20864670201790242No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA34350.20285916259029005No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC33830.19978822330216922No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA33430.19742596231130702No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG33400.19724879273699236No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA33100.1954770969938457No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG32940.19453219259750085No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA32030.18915804884328938No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG31110.18372484856430638No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA30270.17876410048349578No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT29440.17386240892745675No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT29420.17374429587791362No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT29130.17203165665953854No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC28960.1710276957384221No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA28760.16984656524299102No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC28760.16984656524299102No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG28400.16772053035121504No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC28370.1675433607769004No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC25560.15094847731609357No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA25550.150889420791322No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT25450.15029885554360645No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC25230.14899961199863224No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG25190.14876338589954605No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA24330.14368452476919233No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC24090.14226716817467502No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA24060.14208999860036037No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC23230.13718830704432133No Hit
GTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATG23090.1363615156975196No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA23050.13612528959843334No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA22960.13559378087548934No Hit
CTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT22440.13252284158736852No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG22360.13205038938919608No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA22270.13151888066625209No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG22230.13128265456716587No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA22010.12998341102219169No Hit
GAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAA21550.12726681088270017No Hit
GCATTGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCC21510.12703058478361395No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG21100.12460926726798019No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT20880.12331002372300601No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG20870.12325096719823445No Hit
AGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGA20510.12112493230645849No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA20460.12082964968260072No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC20400.12047531053397137No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA20130.11888078436513941No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA20080.11858550174128164No Hit
ACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTT19870.11734531472107898No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA19850.11722720167153587No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT19770.11675474947336344No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC19670.11616418422564789No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG19120.11291607536321238No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT18950.11191211444209596No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG18940.1118530579173244No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT18900.1116168318182382No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA18890.11155777529346664No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG18790.1109672100457511No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG18740.1106719274218933No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA18720.11055381437235022No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA18660.11019947522372088No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC18530.10943174040169068No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT18410.10872306210443201No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA18320.10819155338148802No Hit
ACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA18150.1071875924603716No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT18140.10712853593560004No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA18030.10647891416311293No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA18020.10641985763834139No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC17980.10618363153925517No Hit
GGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAG17930.10588834891539739No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG17840.1053568401924534No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT17660.10429382274656541No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG17470.10317174877590587No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG17350.10246307047864724No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC17200.1015772226070739No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT17200.1015772226070739No Hit
GACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG17150.10128193998321613No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC17090.1009276008345868No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA17010.10045514863641436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)