FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178883
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGT16570.9263037851556605No Hit
GGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAG15020.8396549700083294No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGT13240.7401485887423623No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACA9710.5428129000519892No Hit
TAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTA8670.48467434021119954No Hit
GTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAACAACG8640.48299726636963825No Hit
TATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAA8330.46566750334017204No Hit
GTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGA7420.414796263479481No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAG6740.3767825897374262No Hit
TAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAA6700.3745464912820112No Hit
AACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAA5860.32758842371829633No Hit
ATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTAT5210.2912518238178027No Hit
GTAGAAACACCGGGTTATTAGTAGAAACAACGCACCCGGGTTATTAGTAG5180.28957474997624144No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTAC4980.27839425769916654No Hit
ATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAG4970.27783523308531277No Hit
GGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTA4840.27056791310521405No Hit
GGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATT4810.26889083926365276No Hit
CAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGAAAC4750.2655366915805303No Hit
CACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACG4630.25882839621428533No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGG4620.25826937160043156No Hit
AGTAGAAACAACGCACCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGT4350.24317570702638036No Hit
CAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGG4300.24038058395711165No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAG4170.23311326397701287No Hit
GTAGAAACACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAG4110.2297591162938904No Hit
AGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAA4010.22416887015535297No Hit
GGTTATTAGTAGAAACACCGGGTTATTAGTAGAAACAACGCACCCGGGTT3850.215224476333693No Hit
CCCACGTGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAAC3760.21019325480900924No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGGATTCCGGG3750.2096342301951555No Hit
GTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAG3720.20795715635359424No Hit
GTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTCCGGGTTATTAGT3560.19901276253193428No Hit
GTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAA3530.19733568869037305No Hit
TATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTA3350.1872732456410056No Hit
AGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACA3320.18559617179944435No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGG3290.18391909795788308No Hit
GGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGCCCG3280.18336007334402935No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCCG3250.1816829995024681No Hit
AAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACC3210.1794469010470531No Hit
TAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGA3180.17776982720549186No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA3080.17217958106695438No Hit
TATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTA3070.17162055645310062No Hit
ACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGG2970.16603031031456314No Hit
ATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAAC2920.1632351872452944No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTCCGGGTTATTAGT2920.1632351872452944No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGGTGCGTTGTTTCTACTA2890.16155811340373316No Hit
GATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCC2890.16155811340373316No Hit
AACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGAA2810.15708591649290318No Hit
ATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTA2780.15540884265134194No Hit
GGGCTCGTGTTTCTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCC2710.1514956703543657No Hit
GTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA2610.14590542421582822No Hit
GGTTATTAGTAGAAACAACGCACCCGGGTTATTAGTAGTAACAAGAGGAT2510.14031517807729074No Hit
TGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAACCCGGAA2460.137520055008022No Hit
GGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTCCGGG2420.13528395655260703No Hit
CACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGG2340.13081175964177705No Hit
GGAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAA2340.13081175964177705No Hit
CTAATAACCCGGGTGCGTTGTTTCTACTAATAACCCGGTGTTTCTACTAA2300.12857566118636204No Hit
GGCTCGTGTTTCTACTAATAACCCACGTGTTTCTACTAATAACCCGGAAT2270.1268985873448008No Hit
GTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTAT2230.1246624888893858No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGG2230.1246624888893858No Hit
GAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAAT2220.12410346427553205No Hit
GGGCTCGTGTTTCTACTAATAACCCACGTGTTTCTACTAATAACCCGGAA2190.12242639043397081No Hit
GGAATCCTCTTGTTACTACTAATAACCCGGGTGCGTTGTTTCTACTAATA2150.12019029197855582No Hit
AGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAG2140.11963126736470207No Hit
ACCCACGTGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAA2130.11907224275084832No Hit
ACACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAC2100.11739516890928708No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGTGCGTTGTTTCTACTAATAACC2090.11683614429543333No Hit
AGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAG2030.11348199661231084No Hit
GGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTA2030.11348199661231084No Hit
GTGTTTCTACTAATAACCCGGTTACTACTAATAACCCGGGCTGTTGTTTC1980.1106868735430421No Hit
CCCGAGTGCGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATA1970.11012784892918835No Hit
ACACGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAA1930.10789175047377336No Hit
AGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAAC1930.10789175047377336No Hit
AGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAG1910.10677370124606586No Hit
GGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGC1910.10677370124606586No Hit
TATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTT1880.10509662740450462No Hit
TGTTTCTACTAATAACCCGGGTGCGTTGTTTCTACTAATAACCCGGACGT1860.10397857817679712No Hit
AGCACGCACGTGTTTCTACTAATAACCCGGGTGCGTTGTTTCTACTAATA1860.10397857817679712No Hit
GTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAACAACGCAC1820.10174247972138213No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)