FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F04_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F04_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029650
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG35340.34322342543582773No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT29340.28495119700869226No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA23690.23007818190647306No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC19060.1851114456368669No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG17320.16821249939299762No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA17170.16675569368231924No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA16990.16500752682950517No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA16600.16121983198174136No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT16380.15908318360607973No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA16090.15626669256543485No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG15450.15005098819987375No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG15400.14956538629631427No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA14760.14334968193075318No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA14550.14131015393580343No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC14290.13878502403729423No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG14210.1380080609915991No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC14140.13732821832661582No Hit
GTCTTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA13760.13363764385956392No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG13730.13334628271742824No Hit
ATTTTAGTGTCCTCAACATATTTCTCAAGGTCTTGAATTCTCCCTTCGAC13640.13247219929102122No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT13030.1265478560675958No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG12980.12606225416403632No Hit
CTCAATATCAATGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC12590.12227455931627253No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA12410.12052639246345846No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG11770.11431068809789735No Hit
CCCATATCCTCAGCATTTTCCCTTAGTTGCTTTTTTGTTTTTTCAAACAA11430.111008595153693No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG11240.10916330792016704No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTCCTTTGGCATATCC11010.10692953916379352No Hit
CTCATAAGCACTTCTTGTTTTCCGCCCATTCTCACCTCTCCAAAAATTTC10930.10615257611809839No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT10850.10537561307240324No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT10850.10537561307240324No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA10560.10255912203175836No Hit
ATCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG10480.10178215898606323No Hit
CATGAGAACAGAATGGTTTTGGCAAGCACTACAGCTAAGGCTATGGAGCA10320.10022823289467295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)