FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F04_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F04_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446681
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG53960.3729916961652223No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43480.3005500175919916No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA39800.2751124816044449No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG29580.20446802024772565No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG28550.19734827512077643No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT25930.17923785547746876No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG24480.1692149133084626No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA24290.16790156226562733No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA24080.16644996374459883No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA23820.1646527465280874No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC23510.16250991061609296No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA22630.15642702157559268No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC21880.15124274114334813No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC21440.14820129662309797No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA21320.14737181175393885No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA21030.14536722332013763No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG19890.13748711706312586No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA19510.1348604149774553No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG18960.1310586093271426No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT18610.1286392784587618No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG18470.1276715461114095No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT18410.12725680367682993No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG17710.12241814194006834No Hit
ATTTTAGTGTCCTCAACATATTTCTCAAGGTCTTGAATTCTCCCTTCGAC17430.12048267724536371No Hit
GTCTTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA16650.11509102559582934No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAACAA16490.11398504577028384No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA16190.11191133359738602No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA15610.10790215672978355No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA15600.10783303299068696No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC15100.10437684603585724No Hit
ATCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG15070.10416947481856746No Hit
CTCAATATCAATGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC15010.1037547323839879No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT14790.10223401012386282No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGAATTACAGCTTGTTGGCTT14790.10223401012386282No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTCCTTTGGCATATCC14720.10175014395018667No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC14700.1016118964719935No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT14610.10098978282012414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)