FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451948
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA63690.2597526538083189No Hit
TCTCTGTACACATCGTGATCATAAGTTCCATTTCTGATTGATCCTATGCA49220.2007383517105583No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46820.1909502159099622No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG42820.17463665624230204No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA42490.17329078756972008No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG41400.16884534256028266No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACG41150.1678257450810539No Hit
GTCCCAGATATGTCAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA39460.1609332661214675No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT39030.15917955845719403No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA38370.1564878211120301No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT37900.15457097785108004No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT37640.15351059647268214No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG37490.15289883798514486No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG36760.1499216133457969No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA35770.145884007328051No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG35400.14437500305879242No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT33050.1347907867540421No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG32280.1316504265180175No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG31610.12891790527368444No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA29810.12157680342323736No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA29330.11961917626311813No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC27650.11276748120270087No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACCATCATTGCTTT27580.11248199390851682No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC27170.11080985404258166No Hit
TGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACGATCAAGAATCCA27160.11076907014341252No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT27150.11072828624424334No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG26460.10791419720157197No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG26430.10779184550406454No Hit
ATCACGTACTGTGTTGTTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC26260.10709851921818897No Hit
CTGCAGGCCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAACTGAT25960.10587500224311447No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT25700.10481462086471656No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC25650.10461070136887078No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA25560.10424364627634843No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATTCCGAATATC25080.10228601911622921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)