FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996016
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT72170.7245867536264478No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA35030.3517011774911246No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT28390.2850355817577228No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT25170.25270678382676587No Hit
CCAATAGGGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT24000.240959984578561No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT22610.2270043854717193No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17480.17549918876805193No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC17470.1753987887744775No Hit
GTATGGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17380.1744951888323079No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA16670.1673667892885255No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT16550.16616198936563267No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATACAGTAACGC15390.15451559011100224No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15040.15100159033589822No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCGTTT14740.1479895905286662No Hit
ATATGGAACTTCTGGTAGTTCATGATTGTAAATGTGAGGCCCTATTGGCC14090.14146359094633018No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG14010.14066039099773497No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG13880.13935519108126776No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG13850.13905399110054456No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13340.13393359142825015No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA13170.13222679153748534No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13110.13162439157603895No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAT13110.13162439157603895No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG13110.13162439157603895No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG12890.12941559171740213No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC12200.1224879921607685No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG12200.1224879921607685No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC12110.12158439221859889No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA12030.12078119227000368No Hit
TCTCAGAGCTGACACTATAAGTTCACAAATAGAACTTGCAGTCTTGCTTT11660.11706639250775087No Hit
TTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATGACC11620.11666479253345327No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA11550.11596199257843248No Hit
GCTTTATGGTCTGGCGGTTAAAGGACAATCTCATCTGAGAGGAGATACTG11480.11525919262341167No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG11370.11415479269409327No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGATTTGAGCCATACTGTA11130.11174519284830767No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA10980.11023919294469164No Hit
GTACATGACCATGAGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTG10890.10933559300252205No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA10700.10742799312460843No Hit
GTAATACCAATAACCAATAGGGTGGTAAATGAAAAGGGAGAAAGTTTCGA10690.10732759313103403No Hit
AGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC10610.10652439318243882No Hit
ATTTACTCTGTCTCCCTTGAAACAAGCTTTGGTCATGTCATATCCTTGTA10590.10632359319529003No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT10500.10541999325312044No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG10480.10521919326597162No Hit
CTGATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCC10280.10321119339448362No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC10200.10240799344588841No Hit
CCATGAGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCA10160.10200639347159082No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA10130.10170519349086762No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10120.10160479349729322No Hit
TCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAGATTTCTTTCT9990.100299593580826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)