FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F01_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F01_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211461
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT65830.5433934728398191No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA35120.2898979001387581No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27780.22930989936943905No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGTGTTCCTAGTT26640.2198997739093541No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT24110.19901589898477953No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC22810.18828505416187563No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA22270.18382762631236169No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG20470.16896953348064855No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19270.1590641382595065No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT19260.15898159329933031No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA18020.14874601823748348No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT16960.139996252458808No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT16310.13463083004735604No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT16300.13454828508717986No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16000.13207193628189434No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT15100.12464288986603778No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC15050.12423016506515686No Hit
AAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAATTT14360.11853456281300018No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA13730.11333423032190057No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG13610.11234369079978637No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT13550.11184842103872927No Hit
CCAATAGGGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT13440.11094042647679125No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC13370.11036261175555795No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA13230.1092069823130914No Hit
CCTTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACT13090.1080513528706248No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG13040.10763862806974389No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT12930.10673063350780587No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT12830.10590518390604402No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC12810.10574009398569165No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC12720.10499718934410601No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG12580.10384155990163942No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG12500.10318120022022996No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTTTGG12420.10252084053882049No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA12310.10161284597688246No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG12190.10062230645476825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)