FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313315
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC99540.4302915945299278No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA71310.3082589271240622No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG61970.2678839673801449No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT60500.26152945016134854No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT56520.24432470286147803No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC51160.22115449041743124No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA48270.20866159602129414No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC45330.1959525615837013No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG43410.18765278399180396No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC41910.18116858274813416No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA40640.1756786256951604No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC38330.16569295577990892No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG37880.16374769540680797No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG37100.1603759107600997No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG37020.16003008669377064No Hit
GTTATAAATCTGTGAGTGATTTCATAACCAAACCTCGTGCCTTGTAACTG35260.1524219572345314No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG34960.15112511698579745No Hit
CTCTTGGACAGGGGAATCAAAAGCTGCTTAGAGTGGTCAGGAGACGTGCC34560.14939599665415215No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT33540.1449867398084567No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT31860.13772443441554652No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG31420.13582240205073673No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT30280.13089440910554764No Hit
ATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAGC29650.12817104458320636No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG27990.12099519520687844No Hit
CCCTTATAGCAACATGAGTTTAGAGCAAGAATCACACCCCGACTTTACAG27870.12047645910738486No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC27430.11857442674257504No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA27220.11766663856846128No Hit
GCTATAAGGGTTACGAGAGCTCATGCCAGGGAAGCACCTGGTACACAGTA26820.11593751823681599No Hit
GTCCAAGAGGGTAGGCCCCCTGTTCCTATTTACAGGATCCATAAAGAGAC26720.11550523815390468No Hit
CTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCCCTTACTTCTCTGAGCCT26700.11541878213732241No Hit
GTGTAGTGCAGTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGT26270.11355997778080373No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT25870.11183085744915847No Hit
AGCGGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG25770.11139857736624714No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC25650.11087984126675356No Hit
CCTAGAAAGTGTGCCTAGCACATAGGAGGCACACAAAAATATGTGTTGAA25520.11031787715896885No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT25360.10962622902631074No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA25330.10949654500143732No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC25300.10936686097656394No Hit
GCACTACACCACCTCTCAGGAGAGAAGGCAAATATTTCCTTGACTCAGCC25070.10837261678586789No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT25030.10819970475270337No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA24160.10443886803137489No Hit
CCATTACAGTCCCTCAAGATCCATCCCTCCCTCCATGATGCCTGGCTGCA24110.10422272798991923No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG23760.10270974769972961No Hit
CACATGACCCACGGCTCAAGCGTGGCTCAGGCCAGCAGTCACCAGGCTTC23550.10180195952561584No Hit
GTATTGAAGAAAGCATTGGCCTCTCACTCCCTCATGGGTTCTGCAGGATG23470.10145613545928678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)