FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E05_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E05_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTAAACAACGTGCATTCAAATAACACAGTACGTGATAGGACCCCTTA11.8518518518518516No Hit
GGACAGTAGAAAACAGTATCTGCAACACTACTGGAGCTGGAAAAAAGAAG11.8518518518518516No Hit
CACGCAAGGTGAGCACTGGCAAGGGGCCTTAGGGTTTGCCAATGTGGAGG11.8518518518518516No Hit
TGAAAGATGAGCCTTCTAAGCCAGGTCTAAACGTATCATCTCTCTATCGT11.8518518518518516No Hit
CGACTCTACTGTTCCTAAAGGTTCCAGCGCAAAATGCCATAAGCACAACA11.8518518518518516No Hit
TATATAACCCCAGCACATTGTCCCGCGAAGAAAAAGGATTATCCAGCACT11.8518518518518516No Hit
ATCCTGGTGTCAGATGTGTCTGCAAAGACAACTGGAAATCATCCAATCAG11.8518518518518516No Hit
TTATTAAGATTGGGTCAGTTTGATAGCGTTTTGTTGAGTACTTTTAAAGA11.8518518518518516No Hit
CTGTACCTACTTCGCGTTACCTTTCTGACATGACCCTCGAGGAAATGTCA11.8518518518518516No Hit
GCTCACTGGCCCCTGGCCCTGGGGGAGCTCCGGCAGGACGCACCTGTTGG11.8518518518518516No Hit
TAACAGTCTTTTCGATCGATCGAGACTTCAGCCAACCAAAAAGCGCAGCT11.8518518518518516No Hit
ATGAGGAGCTACATAGACAATATAGACACAAAAGGAGCAATAGAAAGAAA11.8518518518518516No Hit
CTAGGAGACAGAGTGAGACCCTGTCACAAAAACAAGAAAGCTAAATTTGT11.8518518518518516No Hit
TCATTGGATTGTGCAACCGCATCAAATGTTCAGGCTGTGAACCGTTCTGC11.8518518518518516No Hit
AACTCATTCATCAATAGGGTCCGATAAGGGGTCCTATCACGTACTGTGTT11.8518518518518516No Hit
ATCTGGAACTTATGTTTTAGAGGGAACCAGAGCATAAAAGTTTGGAAAAT11.8518518518518516No Hit
AGCACAGCTCTTCTTGGTCTGAGGTTAGAGGCCGACCCCAGGGAAGGGGC11.8518518518518516No Hit
GACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCA11.8518518518518516No Hit
GGACAGGGCCGAAGGTACAATGTTTCAACCTTTCGACCTTTTCGAAATAA11.8518518518518516No Hit
CAGAAACGATTGAGCTGAGAACTCTCATTGACAGGCACACAGAAGCTCTG11.8518518518518516No Hit
GATCAGAGTCAAAAGCCCCAGGGGATTGTGGCCTGTTATTCTACACTTCA11.8518518518518516No Hit
ATTTGATAGGCATTTTGCTTCTGTGCGGTGTTGCCATTAGCACTTGTCTC11.8518518518518516No Hit
CGCTGGGACCACGAGAGCCATGCGCGGTCACGTCCAGAGGGAACTTCTCC11.8518518518518516No Hit
CTCAGAGACTCTGGTACTCCTTCCGTAGAAGCGCCTCTTTTAAGGCCGTG11.8518518518518516No Hit
GGATGAGGCAACTAGTGACCTAAGGGAGGCATAATCCGGCACATCATAAG11.8518518518518516No Hit
GCCTTGGCTCTCTGGTTCCAGCCAGTCTTCCACGGCTTCCCTCTGATGCG11.8518518518518516No Hit
AGCACATTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCTTGCTATCC11.8518518518518516No Hit
TTCCCTTTGATAGATCCGTTTGGAAACACACTTTTGGTAGAATCTGAAAG11.8518518518518516No Hit
CTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAGCTCTCAGTTA11.8518518518518516No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC11.8518518518518516No Hit
GGCAAAGCGCCGATGAGCTGTTCACCAGCGCCGGGCCCACCACTGGGTGA11.8518518518518516No Hit
ATATCCTATGGCCTTTCAATGATCCACTCACTAGTAAGGCTTGATTTTGT11.8518518518518516No Hit
CAATGGTAACAAAGCGTCCTGGAATCTTTTCTAAGTACCATGCCTCTGTG11.8518518518518516No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT11.8518518518518516No Hit
GTAATAAAAAGCTGAAAACAAGGCTTTGTGGATTTCAGAGTGATCTCCTC11.8518518518518516No Hit
TCCCCAGCCCATCCTGGGGACACCCTGTGACCATGACCATCACTCTTCAG11.8518518518518516No Hit
GTTTAGTGAGATATTTATATGTTTGCCATTTACCAATATTTAAAGAAATA11.8518518518518516No Hit
GATTGGGTGCAGTGGCTCACGCCTGTAATCCCAACACTTTGGGAGGCTGA11.8518518518518516No Hit
GTCTTTTTGTAGCAGATGGTGGGCCCAACATTTTCAATTAGAGAATCTTG11.8518518518518516No Hit
CTCCTATCATTCCCATCACACAGTAGATGTAGTCAACAAGAGAATATTGA11.8518518518518516No Hit
CCTCTGGGGAAATCCAGCAGATAGAAAAACACACCCTAGGGGCTGGAGCT11.8518518518518516No Hit
CTGTGCAGCAATGGACGATTTTCAACTAATTCCCATGATAAGCAAGTGCA11.8518518518518516No Hit
AGTCATAATTGCATGACATTTAAAATTAGGACAAGCTACCAAGAAAGACA11.8518518518518516No Hit
GCAGTTAAAGTGTATAGGAAACTTAAGAGAGAGCTAACGTTCCTTGGGCC11.8518518518518516No Hit
CTGTGGTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCA11.8518518518518516No Hit
GGTTATATGTGTCTGGCTTGTAAGAAAATAACTCTACCAAAGTCCAAGTT11.8518518518518516No Hit
CACGTTCCACCTGGAACTACCCCTCACCGGCAACGGGACCGTCTCATGGC11.8518518518518516No Hit
CCTCCCGTCACTACACGCCTCAGGAACTGAAAAGGCTCACCATTGGTCGA11.8518518518518516No Hit
TCCCTGTGCTGTTAATCAAAAGTATGACGCCCGGTTTTACTATTGTCCAA11.8518518518518516No Hit
GAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAGGTC11.8518518518518516No Hit
GCAGGTGGAGAGGGAGCAACCTCACCTCCCACCTGCAGTCTGGATTCTGT11.8518518518518516No Hit
CTATAGCACTCATGGCTTCTTTCCAGCTGGGCGGGAGAGCCACTGCAGCA11.8518518518518516No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11.8518518518518516No Hit
CTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGA11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)