FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939266
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT80260.4138679273498324No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG76060.39221024861983866No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT73580.37942190498879474No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG62390.32171966094388293No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC56220.2899034995714873No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC56130.28943940645584465No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC55680.287118940877631No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA53810.27747611725261No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC48210.24859921227928503No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA44630.23013861945705233No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT41540.2142047558199855No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG40180.20719179318360656No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG38900.20059135776113232No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA37960.19574416299775274No Hit
GTCATCGAACAAGTCAAGGCCGCTGGAGCACTGCTTACTGAGGGGGAGGC36910.19032974331525432No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA35740.18429653281189895No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC34640.17862428362071012No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT33250.17145662327911695No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA32930.16980651442349837No Hit
AGCACGCACTTGGCTATTTGGGCCTTGGTATAGCATTGGGTCCTCCAAGC32260.166351599007047No Hit
AGCACGCACTTGCTGTGAGTCACAAGACACTGATGTGTGTTTGCTTGCTT32180.16593907179314235No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA31980.16490775375838074No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG29530.15227410783255108No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG28770.14835509930045698No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC28270.14577680421355296No Hit
CGTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAA26140.13479326714334186No Hit
GTGTAGTGCAGTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGT25710.1325759333686044No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA25380.13087425861124777No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC25170.12979137467474808No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG25060.12922414975562918No Hit
CTCTTGGACAGGGGAATCAAAAGCTGCTTAGAGTGGTCAGGAGACGTGCC24480.12623332745482055No Hit
AAGCGGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC24370.12566610253570165No Hit
GCCCAAGGCCACTCAGCACCTAGAAAGTGTGCCTAGCACATAGGAGGCAC23960.12355190056444036No Hit
GAAGCGGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT23080.11901410121148928No Hit
GTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGTTCTCTCATTC22370.11535292218808559No Hit
CCCTTATAGCAACATGAGTTTAGAGCAAGAATCACACCCCGACTTTACAG21450.11060885922818221No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT21440.11055729332644412No Hit
ATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAGC21390.11029946381775373No Hit
GCTATAAGGGTTACGAGAGCTCATGCCAGGGAAGCACCTGGTACACAGTA21040.10849465725692091No Hit
ACCCTATCTCTTTGAGTCTCTTTATGGATCCTGTAAATAGGAACAGGGGG20750.1069992461065166No Hit
CTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCCCTTACTTCTCTGAGCCT20650.10648358708913579No Hit
GTTCAATGACAGCATCCACTATACGGCTGGCAGCCTCCTCTATCAAGTCT20600.10622575758044539No Hit
GTTATAAATCTGTGAGTGATTTCATAACCAAACCTCGTGCCTTGTAACTG20270.10452408282308873No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC20210.10421468741266025No Hit
TTCCCAGACAGGCACGCAAGACTCCTCTGGGCCCGAGTGGGCTGAATCCC20050.10338963298485097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)