FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E04_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E04_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG10.9523809523809524No Hit
CACACACGCAGAGCTAGAATTGAAATCATCTCAAGTGAGAATTCTTGTGA10.9523809523809524No Hit
GGTGAGAGGCAAGATGCCTGTAGGGAAAGCCAGGAGCTAGTTAACCCCAG10.9523809523809524No Hit
CTTCATTATGTATTCAGTAGCTCTACAATGGGACACCTCTGCTGTGAAAT10.9523809523809524No Hit
TCCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACA10.9523809523809524No Hit
ATCTTGAGTTGTATGGAGAGGGAACTTCCCCAATAGGACAGCTCATTAGG10.9523809523809524No Hit
GTGTAATGATAAAAGGAATGAGGGAGGCTGATAAATACAGATCTGTATGT10.9523809523809524No Hit
AAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTT10.9523809523809524No Hit
GTTCTCAAACAAAATGGCAAGACTGGGAAAAGGATACATGTTCGAGAGTA10.9523809523809524No Hit
CCATTCTGTTCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGC10.9523809523809524No Hit
GAATAAAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAA10.9523809523809524No Hit
GATGTGTCTGCAGAGACAACTGGAAAGGCTCCAATAGGCCCATCGTAGAT10.9523809523809524No Hit
GCTGTTAATCAAAAGTATGTCACCCGGTTTTACTATTGTCCAATAGATGC10.9523809523809524No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT10.9523809523809524No Hit
ACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAGGGGGAAATG10.9523809523809524No Hit
GCTTTGTCCATATTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC10.9523809523809524No Hit
GCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGCATGGCTAGAGGAAGGT10.9523809523809524No Hit
CTTATATGGTGTAGAATTAGGCCTTCGTAGGCTTTGGAGCTGTGTTTGAA10.9523809523809524No Hit
GATGGTGGCAACAACCAATCCATTAATAAAACAGGAGAACAGAATGGTTT10.9523809523809524No Hit
GCCGATGTTCTTGTGAGGACAAACGGAACATCAAAGGTCAAAATGAAATG10.9523809523809524No Hit
ATTTAGTAGAGATGGGGTTTCACTGTGTTAACCAGGATGGTCTTGATCTC10.9523809523809524No Hit
GCCCAGAACCATAGCATGCATGGACAAGACATCATTAGCTCCAGAGAATG10.9523809523809524No Hit
GTACTTGGGACATTTGACACCACCCAGATAATAAAGCTTCTCCCTTTTGC10.9523809523809524No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATTTGAAGTTTAAATGGGTCAAC10.9523809523809524No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC10.9523809523809524No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC10.9523809523809524No Hit
ACTCTAGTTCCCTCCCTCTGATAATGTCTATGGCTAAGTTCTTCCCTGAT10.9523809523809524No Hit
GCTCAACTCTCCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGA10.9523809523809524No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT10.9523809523809524No Hit
GTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCTTGCTATCCTCGATATC10.9523809523809524No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTGGCTGCAATGAC10.9523809523809524No Hit
GCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATTGG10.9523809523809524No Hit
TGGCCTTCCCTGACCACCCTTACCAGGTGCTGTTGCATAACCTCCCTTTC10.9523809523809524No Hit
GATAATAAAGCTTCTCCCTTTTGCAGCTGCTCCACCAAAGCAAAGCAGAA10.9523809523809524No Hit
CTATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTG10.9523809523809524No Hit
CAAAGAGAACAGATTCATCGAAATTGGAGTGACAAGGAGAGAAGTCCACA10.9523809523809524No Hit
CCTTCTAAGAAACCAGCAATAGCTCCGAATACACCCCTTTCCGTTAATAG10.9523809523809524No Hit
GTTCAGGACTTGTTGGAGACACACCCAGAAAAAACGACAGCTCCAGCAGT10.9523809523809524No Hit
ACAACACACTTTCAAAGAAAAAGGAGAGTAAGGGACAACATGACCAAGAA10.9523809523809524No Hit
AGCCTTAGCTGTAGTGCTTGCCAAAACCATTCTGTTCTCATGTTTTATTA10.9523809523809524No Hit
GTGTTAATGTATACCCCCTTCATTATGTATTCAGTAGCTCTACAATGGGA10.9523809523809524No Hit
GAACCTCAGTGTTCGCCTAAGAGGGGTCTTGTAGATAAAGAGGGCACAGA10.9523809523809524No Hit
GAATTACTCTTGATTTGGGTGAACGGAATTTGAGAAAGTGGACTCATGGA10.9523809523809524No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG10.9523809523809524No Hit
TAGTGACACCTCCTTGGCCCCATGGAACGGTATTTCTCTTTTGAGCTTCT10.9523809523809524No Hit
GCCCACATCCGCCTTTCCCCGGGCTTGGTCCATGCGGATACAGGCACAGT10.9523809523809524No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCCT10.9523809523809524No Hit
GAGACTGAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAAC10.9523809523809524No Hit
GTGCGGTCCCTTGTGGTCAGCCCTCATGAAGACATGAGAACCTGGCTCAA10.9523809523809524No Hit
CCTATGATCTTGGGCAGCTCTGCCCTGCAGGGTACATCTCCCCTGCTGGC10.9523809523809524No Hit
GAAGAGACGACCAGTCAATAGCCGAAGCAATAATCGTGGCCATGGTGTTT10.9523809523809524No Hit
GAAAAAAAGGCGATCAGTAATCCACAATATCAGGTGCAAGATCCCAATGA10.9523809523809524No Hit
ATCGTAGATATAAACATAAAGGATCATAGCATTGTTTCCAGTTATGTGTG10.9523809523809524No Hit
GAACTGATGATATTCAGCTACAATCAAGACTACTCGTTAAGTAATGAATC10.9523809523809524No Hit
GTACGGTTTCAGGCATCAAAATTCTGAGGGAAGGGGACAAGCAGCAGATC10.9523809523809524No Hit
GTGTGGAATTGTACTAACGTGAGAAAGGTAAGAAGGCTTCAAGAAGGCTG10.9523809523809524No Hit
TTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCTTGCTATCCTCGATA10.9523809523809524No Hit
CATTTTGAGTATGAAATCACACTCAACAAGCAAAAACAGCAAAAACAAAA10.9523809523809524No Hit
TCATTATGTATTCAGTAGCTCTACAATGGGACACCTCTGCTGTGAAATAA10.9523809523809524No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGCAGCTGTGTTTGAATCC10.9523809523809524No Hit
ACATCCAAATGTGCACTGAACTTAAACTCAGTGATCATGAAGGGCGTTTG10.9523809523809524No Hit
GGATTCTCGTTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGAAGTAG10.9523809523809524No Hit
GTTTTGAAACTGTCATTCCTACCTATAGGTAGATAAGGGCAAGAGATTAT10.9523809523809524No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC10.9523809523809524No Hit
CAATTGGAAGTCCAGACTGTTCAAGCTTTTCACAAATGCTTCTAGCTAAA10.9523809523809524No Hit
CTACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAACA10.9523809523809524No Hit
CACAATAACGTTCCGCCCAGGATAGTGCAGTGGGAGGAGCCTTGCAGTGA10.9523809523809524No Hit
CACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAG10.9523809523809524No Hit
CGGTTGTTTAAAGCTTCATCTCTGTACACATCGTGGTCATAAGTTCCATT10.9523809523809524No Hit
CTCAATATCAATGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC10.9523809523809524No Hit
TCCCACCTCTCTGACCATTCCTTCTCAGGCTCCTTTGTGTGCCCCTCTTC10.9523809523809524No Hit
TAACAGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAA10.9523809523809524No Hit
GATAATACCAGATCCAAAATTTCTTTCCATTGTGAATGCATAACAATCTA10.9523809523809524No Hit
GCGAGAGAAAAATAAAAAATAAAAATTTACCAAAACATATTTCACACACA10.9523809523809524No Hit
CCACATATATTACCTTGAAAAGGCCAACAAGATTAAATCTGAGAACACAC10.9523809523809524No Hit
CATTATGTATAGGTTATCATGCGAACAATTAAACAGACACTGTAGACACA10.9523809523809524No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG10.9523809523809524No Hit
TCATAAAGGGAAGATCTCATTTAAGGAATGACACAGACGTGGTAAATTTT10.9523809523809524No Hit
CATATATCCTATATATCCTTTTGGTTCTATTTCTCTGGGGACACCTAGCA10.9523809523809524No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATA10.9523809523809524No Hit
ACAATAGGGAAGAAAAAACAAAGAGTGAATAAGAGAGGCTACCTAATAAG10.9523809523809524No Hit
GTTCACAATGGTGGGGAAAAGAGCAACAGCTATACTAAGAAAAGCAACCA10.9523809523809524No Hit
GGAAGAAATACACCCCAAGCCTGGTGTGTTCACTCTGCTCCCAAAGGTGC10.9523809523809524No Hit
GTTGGAAGCGTTGCATAAAGGTTATATTGACCAAACTAAACTCTGTATGT10.9523809523809524No Hit
CATGTCTGAAGTTCTTCCCTCCGTATTTCCAGTGAATGCTGCCATGATGG10.9523809523809524No Hit
ACATAGTGTTGTGCCTGCGAGTGAACAGAAGTCAACAATTGAAGTTTGGG10.9523809523809524No Hit
CAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAGACAGATGGTGCAGGCA10.9523809523809524No Hit
GATAATATGGGATCGAGCACTCTTGAACTGAGAAGCGGTTACTGGGCAAT10.9523809523809524No Hit
GGACAAGAGCTCTTGTTCGAACTGGAATGGATCCCAGAATGTGCTCCCTG10.9523809523809524No Hit
TCTCACTTATATTGTGTAGAATTAGACCTTCGTCGGCTTTGGAGCTGTGT10.9523809523809524No Hit
TCTGTTCTCTTTGTAATCATACAAATCTGGTAGAAACTTCGGTTTTCCAG10.9523809523809524No Hit
CTTATTAGGTAGCCTCTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGT10.9523809523809524No Hit
CCTTGGTATGACACTGACGGTACTTGTATAATTTGCAAAGATTGAGGCAA10.9523809523809524No Hit
TGTCCAAAGTATGTAAAAAGCACAAAATTGAGACTGGCCACAGGATTGAG10.9523809523809524No Hit
AATCAGACCTAACGAGAATCCAGCACACAAGAGTCAGCTGGTGTGGATGG10.9523809523809524No Hit
CAACCAATCCAATAATAAAACATGAGAACAGAATGGTTTTGGCAAGCACT10.9523809523809524No Hit
ATTTTGTGCGACAATGCTTCAACCCGATGATTGTCGAACTTGCAGAAAAA10.9523809523809524No Hit
GTTCTGGAGCACGGGGCCCAGCACAGGGTGAGAGGCAAGATGCCTGTGGG10.9523809523809524No Hit
GTGCTGGATTCTCGTTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGA10.9523809523809524No Hit
TCTCAGTTCCTTGCGTTTCACTCACTTCTTCGGGAGACAATAGTAAGTTC10.9523809523809524No Hit
GGTCTTTAGTGTGTGATTGGGACAGTAGCCAGACTGCCGGCTGGAGGTGA10.9523809523809524No Hit
GGAATGCAGTGGCGCGATCTCGGCTAACTGCAAGCTCCACCTCCCGGGTT10.9523809523809524No Hit
GTGGATAAACCGAGCGTGAGAATCAGGTGTCTGCGGGCGCCTTTCATATC10.9523809523809524No Hit
GTGGGAATAGACCCTTTCAAACTACTTCAAAATAGCCAAATATACAGCTT10.9523809523809524No Hit
GTAGCCCAGATGGTCTCGAATTCCTGGGCTCTTAAGAGATACTCCCAAAG10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)