FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156783
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT105290.4881807766474421No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT72910.33804977134927344No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC71520.3316049876134966No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA67360.3123170017567832No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT62860.2914525939790883No Hit
TTTTTAAACTATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCG59910.2777748155470439No Hit
GATCTTATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGAA48010.22260004831269534No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC45890.2127705939818702No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC45370.21035959574978105No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA44640.2069749251547328No Hit
CTATAAACACAAGCCAAAGGGGAATTCTTGGACACAGTCAACAGAACACA44310.20544486858436847No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAA43980.2039148120140042No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA43210.20034468001648753No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC39960.18527594106593012No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC38580.1788775226807704No Hit
GCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG36500.16923352975241368No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA35360.16394787978206432No Hit
ATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT34410.15954317147343985No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA32590.1511046776611277No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG32520.150780120206808No Hit
GATCTTCATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGA31640.14669996935250323No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC28390.13163123040194585No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGCATTAATTGATGGCCATCC27830.12903477076738828No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC27660.1282465598068976No Hit
CTATGGACACAGTCAACAGAACACACCAATATTCAGAGAGGGGGAAGTGG27060.1254646387698716No Hit
GCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAACAGATC26920.12481552386123221No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT25660.11897348968347767No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC25450.11799981732051856No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA24710.1145687813748532No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC24450.11336328225880861No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT24380.11303872480448891No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA24110.11178686033782723No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA23400.10849492044401315No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTTTGCCG23080.10701122922426595No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT22900.10617665291315817No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA22860.10599119151068975No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA22100.1024674248637902No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC21630.1002882533847865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)