FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E03_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E03_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC12.631578947368421No Hit
CACACACACACACATTTTTTAAAGCTTTGTAAGTGATTCTAATGCATAGC12.631578947368421No Hit
CTCAGTTAATTGATGCTTAATGCTGGTCCTTATCTTCTGTCTTCCTTACC12.631578947368421No Hit
CCAAAAGATAATAACCATTGGTTCGGTCTGTACGACAATTGGAATGGCTA12.631578947368421No Hit
CCATTGTATCTCCCATCCTAGAGACTGTCCTAGGGTACCTTTCTCTTGCC12.631578947368421No Hit
GAGTGGACAAGCAAGACTCTGCTGGGCAGTGCATGGAAAGGACTCTGTAT12.631578947368421No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT12.631578947368421No Hit
ACAATGGGACACCTCTGCTGTGAAATAAATCCTTCTCATGCTTGCAATGT12.631578947368421No Hit
TAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTAT12.631578947368421No Hit
CTCTGTCACAAAATAAATAGTATGTGTATGCAGTGTCCTGACTGTTATCC12.631578947368421No Hit
GCTGAAATGAGAAAGTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGT12.631578947368421No Hit
GTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCA12.631578947368421No Hit
GTCTCAGCTATGAACACAGCAAAAACAATCAATGGAATGCGAAAAGGAGA12.631578947368421No Hit
AGACAGGGTTTCATCATGTTGGTCAGGCTGGTCTCGAACTCCTGACTTTG12.631578947368421No Hit
GTCACGTCAGAGGGAACTTCTCCTGACCCACTCTAGCCCCGATTGTCCTT12.631578947368421No Hit
GAATGTACTATTCATCTATGGCTATCAGCCATATAATGTTGACATATGTC12.631578947368421No Hit
CTACAGGTCGGCTGCATATCCTGACCCATGCTCATTTTGATGGTGATAAC12.631578947368421No Hit
CGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGGGA12.631578947368421No Hit
TTCTCAAGGTGACCTACCGCAAAGTGGCACACTCTGATCTGTTCTAACTG12.631578947368421No Hit
GCATTCAAACCAAAATTTTCACTCCAATGAGACGTCTCTGCCACTGCAGC12.631578947368421No Hit
GTAGTAACAAGAGATATGTATACTAATCTATCACAAAAACACCTCACACA12.631578947368421No Hit
ATACAGGCTATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCC12.631578947368421No Hit
GAGCCGGTCAGTGGCCACAAGCGACTCAGACCAAGGGGACAGCAGGTGTC12.631578947368421No Hit
GTGCAAGCTGCTGGTGATCTACCATTCTGGGGTCTGCATGGTGGGAGCCC12.631578947368421No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT12.631578947368421No Hit
GGGATGGGCTCCAATCCTCAGACGATTTTGCCCTCATAGTGAATGCACCA12.631578947368421No Hit
AAACAAGGAAACCTATATTGATAGAAGCAGAAAAGAAGCTTTAAAAAAGT12.631578947368421No Hit
GCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATT12.631578947368421No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG12.631578947368421No Hit
GTCACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC12.631578947368421TruSeq Adapter, Index 12 (95% over 23bp)
CTCCAAACCTACCTGGCTCAGCCCAAGTCCTCTGATTTTCATGTCTCTGC12.631578947368421No Hit
GATACAGCATTGCACACACCAGGACGCGGAAGCCCAGGGCAGATGACTCT12.631578947368421No Hit
AGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGTGAG12.631578947368421No Hit
CCTGAAATCTCAGTAGAACCAAATTATTTCTAAACCTAAGATGGTATCTT12.631578947368421No Hit
GTTTGCTTTTATCGTTTGTGGAACAATAGGTTATCATCTATTCTTATACT12.631578947368421No Hit
TGGCAAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTG12.631578947368421No Hit
CCCCATAGCGTCTCCTCATGTGTCCAAATTTCCTCTTCTTATAAGCACAC12.631578947368421No Hit
TCCTAGCTCTGTTATCTTCTAGCTGTGCGACTATCCACAAGTATCTTAAC12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)