FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_E01_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_E01_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436822
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT46210.3216125588277462No Hit
TTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT32870.22876876885237005No Hit
GTATAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27170.18909788408028272No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC19220.13376743952973996No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT19120.1330714590951419No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG17230.1199174288812393No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT17100.11901265431626185No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT17060.11873426214242266No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC16970.11810787975128442No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT16780.11678551692554817No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16610.11560235018673154No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT15870.11045209497070618No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT15710.10933852627534936No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA15440.10745937910193468No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC14930.10390987888548478No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC14840.10328349649434655No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA14710.10237872192936913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)