FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199624
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC78390.35637909024451453No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG73070.3321931384636647No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC64760.29441395438493123No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA58460.2657726956970828No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA55380.2517703025608013No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG53880.2449509552541707No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT46550.21162707808243592No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG44400.2018526802762654No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG44190.2008979716533371No Hit
ATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAGC42380.19266929257000287No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT41910.19053256374725863No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC39860.1812127890948635No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA37160.16893796394292843No Hit
AGCGGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG35580.16175491811327755No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG33150.150707575476536No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC32660.14847992202303667No Hit
GCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGT32480.14766160034624098No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC32150.14616134393878227No Hit
AGCACGCACTTGGCTATTTGGGCCTTGGTATAGCATTGGGTCCTCCAAGC31760.1443883136390583No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA31690.14407007743141556No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT30810.1400693936781923No Hit
CACATGACCCACGGCTCAAGCGTGGCTCAGGCCAGCAGTCACCAGGCTTC30790.1399784690474372No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA29780.13538677519430595No Hit
TACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGATGGCG29220.13284088553316387No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG28940.1315679407025928No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC28670.1303404581873993No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT28080.1276581815801246No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC27620.12556691507275788No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT27480.12493044265747236No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA27100.12320287467312595No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG26190.11906580397377006No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG25860.11756554756631134No Hit
GTTATAAATCTGTGAGTGATTTCATAACCAAACCTCGTGCCTTGTAACTG24830.11288292908242499No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC24440.11110989878270103No Hit
GTGCAGAGGAGGAGGTAAGAGGGGTTAGGTTCTGAAGGTGGAGTAGATGT24290.11042796405203799No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC24270.11033703942128291No Hit
TCCTTGGCCTGGCTCACCTCTTCATGGAATCCGCGGGCTGCCCTGTGGAT23580.10720013966023284No Hit
GTGGATAAGGGATTGGGCAGTGTGTGAAGGGCTAGTAAGGAGGCTGCTCA23450.10660912956032487No Hit
GGATAAGGGATTGGGCAGTGTGTGAAGGGCTAGTAAGGAGGCTGCTCAGT23280.10583627019890673No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT23250.10569988325277412No Hit
CTCTTGGACAGGGGAATCAAAAGCTGCTTAGAGTGGTCAGGAGACGTGCC23220.1055634963066415No Hit
GTGTGTGAAGGGCTAGTAAGGAGGCTGCTCAGTAACTTGGGCTTGACCAG22960.10438147610682554No Hit
AAGCAGAGCTGTCAGAGTAGAGTCAATGTCGTAGGGTGAAGGTATGGGCA22550.1025175211763465No Hit
GAAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGG22540.10247205886096897No Hit
GGATTGGGCAGTGTGTGAAGGGCTAGTAAGGAGGCTGCTCAGTAACTTGG22410.10188104876106098No Hit
AGCACAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG22400.10183558644568345No Hit
GGTAAGAGGGGTTAGGTTCTGAAGGTGGAGTAGATGTGGAGAATGGCGGG22220.10101726476888777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)