FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D08_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D08_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996972
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA153730.3846161544289027No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT125170.3131620636822074No Hit
GTATAGGAAACTTAAGAGAGAGATAACTTTCCATGGGGCCAAAGAAATAG98630.24676179868160197No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG95510.2389558896084336No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG90870.22734710175602932No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA75550.18901808669162556No Hit
TTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACGA70550.17650861702308646No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG66910.16740172310439003No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA65910.16489982917068222No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT64660.16177246175354745No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC63140.15796958297431155No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT62950.15749422312690706No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA61980.15506738601121048No Hit
GGTATGTGCAACATGTGAGCAAATTGCTGACTCCCAGCACAGGTCTCATA61070.1527906625315364No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA57940.1449597345190309No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT56560.14150712089051412No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA56260.14075655271040177No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA54920.13740401483923328No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT51550.12897263228263794No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC50470.1262705868342335No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA48530.12141691260284034No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG48520.12139189366350328No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCCCTTTTAAGGCCGTG48290.12081645805875048No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT47950.1199658141212898No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG47280.11828954518570559No Hit
GTGCTGGGAGTCAGCAATTTGCTCACATGTTGCACATACCAGGCCAAATG46690.11681342776481797No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT46580.11653821943211011No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG45840.11468681792116632No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG45210.1131106247429304No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC43310.10835702626888555No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC43290.1083069883902114No Hit
AATATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGAGAGATAAC41820.1046292043076609No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG41220.1031280679474362No Hit
ATCTACAAGTCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT41020.10262768916069465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)