FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588756
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG72720.4577166034306086No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC51990.327237159135827No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG50950.3206911571065664No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA47660.2999831314563092No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT41130.25888179179181703No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA38370.24150970948339456No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT37060.23326426461961433No Hit
GTCAATGAGGTTTCAACATTTTGTTAGCTGTGTGCAGCATTCCCAGAGCT35420.22294172295808795No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA34140.21488510507592104No Hit
AAGCAGAGCTGTCAGAGTAGAGTCAATGTCGTAGGGTGAAGGTATGGGCA33700.21211564267892613No Hit
AGCACGCACTTGGCTATTTGGGCCTTGGTATAGCATTGGGTCCTCCAAGC32370.20374431316073707No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC32200.2026742935982618No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA31350.1973241957858853No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC29130.18335099914650205No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT28820.18139978700316473No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC28450.17907092089660087No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA27910.1756720352275617No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC25250.15892937619118352No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC25210.15867760688236582No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA23660.14892154616567932No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG20580.12953530938671515No Hit
AGCACGCACTTGCTGTGAGTCACAAGACACTGATGTGTGTTTGCTTGCTT20580.12953530938671515No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA20460.12878000146026197No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC20140.12676584698972027No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG19460.12248576873981909No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC19140.12047161426927734No Hit
AGCACGCACTTTGGTTTTTAGAATTTTCAGCTGTTCTGCTCTGGTTTCTC18360.11556211274733187No Hit
GCACAGCAGGTTGTCAAGTGTTGGGGTTGGGGGCCTTATCCAGCCACTCC18310.11524740111130974No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG18070.11373678525840342No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG18050.11361090060399456No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG17980.11317030431356356No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA17900.11266676569592812No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT17870.11247793871431486No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT17430.10970847631731996No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAATGAGGTTTCAACATTT17390.10945670700850225No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT17030.1071907832291428No Hit
AGCACGCACTTGTGGTGACCCTACTTGTAAGGACATCTTGCAGTTCTCCA17000.1070019562475295No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA16900.10637253297548523No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG16870.10618370599387193No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG16840.10599487901225865No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT16690.10505074410419221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)