FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278621
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA78010.34235618823841263No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG65550.28767399229621776No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCTGCTGT63770.2798622500187613No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG44790.19656625652093962No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG41030.18006504811462723No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG39820.17475481881365967No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT39560.17361377780684017No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG39040.17133169579320123No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA38220.1677330280024629No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC38040.16694307653620327No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA35690.156629821282258No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCT35150.1542599668834791No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA35090.1539966497280592No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA32430.1423229225044446No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA31710.13916311663940603No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG31640.13885591329141617No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA31530.1383731651731464No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA30930.1357399936189476No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT30130.13222909821334922No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACCCCTTCCG29940.1313952605545196No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC29140.1278843651489212No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC27860.12226693249996379No Hit
GTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCGACAGGAATGCGA27150.11915101282749523No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC27150.11915101282749523No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG27090.11888769567207536No Hit
ATATACAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGT26970.11836106136123559No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA25770.11309471825283803No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC25390.11142704293517877No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG25360.11129538435746884No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG25260.11085652243176905No Hit
CTCATAGACTCAGGTACCCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG25160.11041766050606924No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC24950.10949605046209965No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG24920.10936439188438973No Hit
GTCCTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA24480.10743339941131061No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG24140.10594126886393129No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT23630.10370307304286233No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT23370.10256203203604286No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT23300.10225482868805301No Hit
GAGTAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA23150.10159653579950328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)