FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D03_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D03_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995917
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT128790.6452673132199385No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC108170.5419564039987634No Hit
TGGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG106700.5345913682783403No Hit
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA102760.5148510684562535No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA100060.5013234518269046No Hit
CTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT96340.48268540224869066No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT95620.47907803781419767No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG85390.4278234014741094No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG69670.34906261132101185No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT64780.3245625945367468No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC58120.29119447351768635No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC52520.2631371945827407No Hit
GCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGC52050.26078238724355773No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTAC51570.25837747762056235No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA51530.25817706848531274No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTAC49020.2456013952483996No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA48730.24414842901783995No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT48130.24114229198909576No Hit
CCAGACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAAC47330.2371341092841035No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG47250.23673329101360427No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG46600.2334766425657981No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC46490.23292551744386164No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG46400.23247459688955002No Hit
GACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAG45940.23016989183417946No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT45360.2272639593730601No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT44240.22165250358607097No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA43720.219047184827826No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG43390.2173938094620167No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC42660.21373634274371128No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC42650.21368624045989887No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG42390.2123835810807764No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA40440.20261363573735783No Hit
TTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGA40250.20166169234492218No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG39850.19965760099242605No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT39190.19635085026080742No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC39090.1958498274226834No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA38400.19239276983962758No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA38190.19134062187956713No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT37950.19013816706806946No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC37870.18973734879757023No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA37770.1892363259594462No Hit
GGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT37620.18848479170226015No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA36310.18192139252283537No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC35860.17966678975127723No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA34090.17079868551648192No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT33580.16824346904204934No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT33150.166089070838116No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT32840.16453590003993152No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT32570.16318313837699663No Hit
ACTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGG32470.1626821155388726No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA32390.1622812972683734No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT31370.15717086431950827No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT30830.1544653409936385No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG30370.15216063593826795No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA30140.1510082834105827No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG30030.15045715828864628No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA29860.14960541946383543No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA29640.14850316921996254No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGT29490.1477516349627765No Hit
TGGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG29280.14669948700271604No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA29060.14559723675884317No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA28660.14359314540634704No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA28490.1427414065815362No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT28400.14229048602722458No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC28030.14043670152616566No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT27390.13723015536217187No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA27260.13657882567261062No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT27030.13542647314492537No Hit
CTCATACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT26860.13457473432011452No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC26720.13387330234674089No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG26660.13357268864386646No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT26460.1325706429676184No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG26370.13211972241330677No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC26120.1308671653179967No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA25790.12921378995218738No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT25650.12851235797881375No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG25400.12725980088350367No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG25090.12570663008531918No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC24840.1244540729900091No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC24610.12330172046232384No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT24060.12054609485264167No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA23750.11899292405445719No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA23430.11738965097246028No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC23400.11723934412102308No Hit
TCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACCAC23310.11678842356671144No Hit
GAGCAAAAGCAGGAAGGTGGGCAAGCTATGCAGGTAACATTGGAGCTAAA23240.11643770758002461No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG23230.11638760529621223No Hit
AGCGAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG22970.11508494591708973No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA22520.11283034314553161No Hit
AACTAGCAACGTACTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAA22470.1125798317264696No Hit
GCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTACAT22350.11197860432072075No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC22320.11182829746928355No Hit
AGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG21810.10927308099485099No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG21790.10917287642722617No Hit
TTGTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCA21730.10887226272435176No Hit
GTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAG21640.10842134217004012No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG21530.1078702170481037No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT21440.10741929649379209No Hit
GCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTACAT21420.10731909192616727No Hit
GCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACC21240.10641725081754402No Hit
CAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCT21190.10616673939848201No Hit
GCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACT20830.1043630571812355No Hit
GCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCACCTGT20490.1026595795316138No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG20480.10260947724780138No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGTGGGGAAAG20470.10255937496398898No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG20250.10145712472011612No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG20150.10095610188199208No Hit
GTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACCACTTC20040.10040497676005566No Hit
GAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTA20030.10035487447624325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)