FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D03_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D03_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686141
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA47580.2821828067759458No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG45320.26877941998919425No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCTGCTGT41030.2433367079028385No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG29600.17554878269373675No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA28870.17121937014757366No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA28700.1702111507875083No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG27760.16463629079655853No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG26150.15508786038652758No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG25550.15152943911570857No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA24230.1437009123199068No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA24060.14269269295984144No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC23800.1411507104091532No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCT23150.13729575403243266No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT23140.13723644701125232No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT22380.13272911340154828No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA21700.12869623596128674No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG21300.1263239551140741No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA20470.12140147235610782No Hit
GTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCGACAGGAATGCGA20270.12021533193250149No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA20240.12003741086896054No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC20130.11938503363597705No Hit
ATATACAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGT19540.11588591938633838No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT18800.11149719981899497No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG18490.10965868216240517No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC18480.10959937514122484No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC18390.109065611950602No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC18320.1086504628023398No Hit
GTCCTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA18150.1076422434422744No Hit
GTATATACATTGAAGTTTTACATTTGACTCAGGGAACGTGTTGGGAACAA17860.10592233982804522No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA17720.1050920415315208No Hit
CTCAATATCAATGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC17670.10479550642561922No Hit
CTCATAGACTCAGGTACCCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG17480.1036686730231932No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACCCCTTCCG17060.1011777781336199No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT17040.10105916409125927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)