FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_D02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_D02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407031
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCG8470.20809225832921818No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC8320.20440703533637486No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA7020.1724684360650663No Hit
GCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGG4640.11399623124528598No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCCG4480.11006532671958646No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCC4180.10269488073389987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)