FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C08_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C08_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462086
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC101740.41322683285636647No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC72960.2963340841871486No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA72900.2960903883942316No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC63670.25860185225049004No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA57350.23293256206322605No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC55900.22704324706773038No Hit
GGCAAGGGGCTGGAGCTCAGGAGAGGCTTGGTCTGGAAGGAAAAAGTTGA52100.21160918018298305No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG47650.1935350755416342No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG45960.18667097737447028No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA45730.1857368101682882No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA43720.1775730011055666No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG39650.16104230315269247No Hit
AGACAGAATCGGGGAGACTCTGCCAACAGAACATGTGGGCAAAGCGCCGA37130.15080707985017583No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA36510.14828888999003284No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA35760.14524269257856956No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA35110.14260265482196804No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT34750.14114048006446567No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT33140.13460130962119113No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC32340.13135203238229695No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA32000.12997108955576694No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA31810.12919938621152957No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31790.12911815428055723No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT31300.12712797197173453No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA31200.12672181231687277No Hit
GGAATGGTCAGAGAGGTGGGAGGAGAACCAGAGCCGACTGCATGACAGAG30420.12355376700895093No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT29590.12018264187359823No Hit
GTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGTCCTCCTGT29170.11847677132317881No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG27680.1124249924657384No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC27350.11108466560469454No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC26720.1085258597790654No Hit
GCCAGCAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG26550.10783538836580038No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT26410.1072667648489939No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG26410.1072667648489939No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT26190.10637321360829802No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC26110.1060482858844086No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT25460.10340824812780708No Hit
GTTCGAGACCAGTCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT25360.10300208847294531No Hit
CTTATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAA25270.10263654478356972No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG25090.10190545740481852No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA25010.1015805296809291No Hit
GTTCTGGAGCACGGGGCCCAGCACAGGGTGAGAGGCAAGATGCCTGTGGG24720.10040266668182997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)