FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C07_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C07_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440423
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT34570.23999894475442282No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG25190.174879184795022No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT20520.14245815291758046No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC20370.14141679215063907No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT17960.12468559582844761No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG17920.12440789962392992No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17460.12121439327197636No Hit
GTTTCAAGCAGCCTCACAGAATGCCATGGAGGCACTAATGGTCACAACTG17150.11906224768696418No Hit
GTAGAAGTACCATACATTTGTGCAGAAGGAGAAGACCAAATTACTGTTTG17100.11871512743131706No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG16580.11510507677258694No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA16010.11114790585820972No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG15610.1083709438130327No Hit
CCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAACCCCCAAAC15580.10816267165964444No Hit
CCTCTATGGAGACTCAAATCCTCAAAAGTTCACCTCATCTGCTAATGGAG15570.10809324760851499No Hit
ATGCATTACTGTCGGGGCCATCAACTCCGATATATGTCCATTCTTTACCA15560.10802382355738556No Hit
GTATTAGTAGTTGAAAACTTGGCTAAAAATATCTGTGAAAATCTAGAACA15400.10691303873931476No Hit
GTACAAACGCATCAAATGTTCAGGCTGTGAATCGTTCTGCAGCAAAAGGG15160.10524686151220856No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACGTAC15110.10489974125656143No Hit
AAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAATTT15000.10413607669413777No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT14830.10295586782493754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)