FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759529
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31480.17891151552489332No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC24080.13685480603047748No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT22930.1303189660414804No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC22010.12509029405028277No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20810.11827028710524237No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA18750.10656260851625635No Hit
GTATTATTATCACTATATGTGTCAGCTTTATTATCATACTTACTATATTC18680.10616477477779565No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18480.10502810695362225No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18150.10315260504373613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)