FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C04_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C04_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748231
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA38690.2213094265002737No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG38370.2194790047768287No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT38170.21833499119967556No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG36470.20861087579387394No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA36350.20792446764758202No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA35110.20083158346923263No Hit
ATATGGGATCGAGTACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG28350.1621639245614567No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT26240.15009458132249112No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT26080.14917937046076862No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA25030.1431732991807147No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC24630.1408852720264084No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG24280.13888324826639042No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG24250.13871164622981746No Hit
GTATATACATTGAAGTTTTACATTTAACTCAGGGAACGTGTTGGGAACAA23360.133620785811486No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG23270.1331059797017671No Hit
CTCAATATCAATGCGGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC21870.12509788466169516No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT20940.11977822152793309No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA20660.1181766025199187No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG20560.11760459573134213No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAACAA20480.11714699030048088No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT20320.11623177943875838No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA20300.11611737808104305No Hit
CTCATAAGCACTTCTTGTTTTCCGCCCATTCTCACCTCTCCAAAAATTTC19920.1139437522844521No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA19160.10959650069127022No Hit
GGATACTGCAGGTCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAA19050.10896729322383597No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT18870.10793768100439817No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC18800.10753727625239456No Hit
GTCCTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA18770.10736567421582158No Hit
GGTCTATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTCGTACCCACTG18610.10645046335409909No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAGAGACT18490.10576405520780721No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA18170.10393363348436219No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGACTCTTATTCCTCTCAT17980.10284682058606671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)