FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C04_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C04_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667539
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA33690.20203425527079125No Hit
GTATATACATTGAAGTTTTACATTTAACTCAGGGAACGTGTTGGGAACAA31930.19147977948341838No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT31630.18968072111057072No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA31540.18914100359871644No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG29080.1743887249413657No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG29060.17426878771650917No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT28770.17252969795608977No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA28580.17139029431995292No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGACTCTTATTCCTCTCAT22550.13522922102571514No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT22480.13480944073871734No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAGAGACT21370.12815292475918105No Hit
ATATGGGATCGAGTACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG20850.1250345569129118No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA20410.12239593796606857No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG19820.11885778983280151No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT19080.11442011251311063No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC19020.11406030083854111No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT18460.11070205854255882No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG18110.1086031571075699No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC17790.10668416150986573No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG17240.10338588782631171No Hit
CTCAATATCAATGCGGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC17030.10212654696531835No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT16940.10158682945346406No Hit
CTCCAGTACAGAGAGGGTGGTGGTTAGCATTGATCGGTTTTTGAGAGTTC16700.10014758275518594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)