FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435989
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC59680.4156020693751833No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG52750.3673426467751494No Hit
GTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAGCTATTCTCTGTCGCT34990.24366481915947827No Hit
GTCTTATACAGTCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG34450.2399043446711639No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT31890.22207691005989602No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA31010.21594872941227264No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT27480.191366368405329No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT27370.1906003458243761No Hit
ACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAGCT24410.16998737455509758No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA24200.168524967809642No Hit
GTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCG23410.16302353291007102No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC21280.14819055020616453No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20260.14108743172823748No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT19860.13830189507022686No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTT19810.13795370298797552No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT19720.13732695723992314No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC19710.1372573188234729No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG19330.13461105899836281No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18310.12750794052043574No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG18210.12681155635593308No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17910.12472240386242513No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT17550.12221542087021559No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC17530.12207614403731504No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA16860.11741037013514727No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16600.11559977130744037No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG16570.11539085605808959No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTCGCTTTCAGT16490.11483374872648747No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACGGTAC16310.11358025723038269No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT16240.11309278831523083No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA16160.1125356809836287No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC16020.111560743153325No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT15790.10995905957496889No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG15630.10884484491176463No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC15610.10870556807886411No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC15540.10821809916371225No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG15480.10780026866501066No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC15160.10557183933860216No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG14620.10181136485028786No Hit
GCATAAATGTTATTTGTTCGAAGCTATTCTCTGTCGCTTTCAATCTGTGC14370.10007040443903122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)