FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C02_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C02_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGTACACATCGTGATCATAAGTTCCATTTCTGATTGATCCTATGCA27120.27497987333891677No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG20450.20735023634885133No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATTCCGAATATC20070.2034972735218311No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG18540.18798402845514442No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT17160.17399168976754467No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTGC15490.15705893208037686No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG15170.1538143318049914No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA14420.14620979990955676No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA13810.14002478063460325No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACG13550.1373885429108526No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT13360.13546206149734247No Hit
CCATTATATGATGCGATCAAGTGCATAAAAACATTCTTTGGATGGAAAGA13230.1341439426354671No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA13090.13272443001498602No Hit
GTCCCAGATATGTCAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA12980.13160909867032225No Hit
AATCAATGGGAAGCTGAATAGATTGATCGGGAAAACCAACGAGAAATTCC12160.12329481046464703No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG12010.1217739040855601No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA11940.12106414777531954No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC11630.11792094125853989No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG11430.115893066086424No Hit
ATCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG11370.11528470353478922No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA11300.11457494722454864No Hit
ATGCTGTACCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTT10390.10534811519142127No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG10120.1026104837090648No Hit
ACACTAAGTGGAATGAAAATCAAAACCCTCGAATGTTTCTGGCGATGATT9870.10007563974391992No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)