FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_C01_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_C01_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA112460.32461497073673684No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT103860.2997911333871376No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG98230.2835401794012952No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG73070.21091602269014192No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTATGTG63300.1827149888639111No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT61030.17616265040070292No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA59740.172439074798263No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT57570.1661753856065618No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG56230.16230748536836842No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG53010.15301297882584403No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT52340.15107902870674733No Hit
AGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA51200.1477884270115679No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC49440.14270819983304525No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT49320.1423618207072369No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA48710.1406010601510444No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA48510.14002376160803046No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG45570.13153747302572558No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT45450.1311910938999172No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA44240.1276984377146829No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA43800.12642838092005224No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT43610.12587994730418897No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG42840.12365734791358533No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC42690.12322437400632488No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA42070.12143474852298168No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC41620.12013582680120033No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA40540.11701841466892504No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG39930.11525765411273255No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC39810.11491127498692419No Hit
TACTAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA39780.1148246802054721No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGT39510.11404532717240329No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA38780.11193818749040241No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA38690.11167840314604614No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC38150.1101196970799085No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC36920.1065693110403728No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC36860.1063961214774686No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA36690.10590541771590677No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC35030.1011138398088911No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG34890.10070973082878135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)