FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC90420.4947618281564458No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTGTAGTAAGGACAATT43040.23550706794794768No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC33460.18308704678295376No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT33060.180898319385668No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA30510.16694518222797128No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30280.16568666397453197No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA30150.1649753275704141No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29960.16393568205670336No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC28710.15709590894018535No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG27710.15162409044697098No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26610.14560509010443512No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA25360.13876531698791714No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC24010.1313783620220777No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA23880.13066702561795981No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22000.12038000685071674No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG21790.11923092496714173No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT20980.11479875198763807No Hit
GCATTATTGTCAGGACCATCAACTCCGATATATGTCCATTCCTTACCATC20370.11146094270677728No Hit
GGAATGATATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG19700.10779482431632363No Hit
GGCGACATGGCAAATAGAATCCCCTTGGAATACAAAGGAATACAACTTAA19530.1068646151724772No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT19450.10642686969302004No Hit
CAATAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACAT19430.10631743332315574No Hit
GTAATATTCAGTGAATCAAAAGTGGGGAGAGAAAATTCTCCTGCATCAAA19390.10609856058342718No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT18980.10385511500120928No Hit
GTTTAATATGCTATCCACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA18680.10221356945324496No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACA18440.1009003330148735No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA18360.10046258753541634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)