FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B06_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B06_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612165
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA25030.15525706115689153No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA18730.11617917520849291No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC17250.10699897343013898No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA16750.1038975539104248No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT16650.10327727000648196No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA16400.10172656024662488No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT16350.10141641829465346No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT16270.10092019117149918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)