FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B06_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B06_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667008
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA53100.31853476408031633No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG47160.2829020616577725No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43770.2625662264368257No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG37020.22207451913848045No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG31630.18974114101432027No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG28390.17030512151111452No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA27960.16772564978692364No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA26950.16166689062080086No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26310.15782767689177257No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG24240.14541021998694667No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG24050.1442704534111414No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA23580.14145103082888624No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA23560.1413310553998541No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC22950.13767180481437402No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT22910.13743185395630975No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA22810.1368319768111491No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA22730.13635207509502054No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT22410.1344324682305064No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAACAA22180.13305275079663686No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA21360.12813375820631934No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT21130.1267540407724498No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA20960.12573424962567667No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA20440.12261488847084118No Hit
ATATACAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGT20430.12255490075632511No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG20230.12135514646600376No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA19750.11847573616923254No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG18830.11295686643375435No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG18400.11037739470956347No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG17910.1074379966982762No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA16920.10149921296118555No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC16900.10137923753215342No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG16860.10113928667408914No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC16790.10071937267247667No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGAATTACAGCCTGTTGGCTT16700.10017948324183208No Hit
GTCCTGAATTCTCCCTTCGACTTCAGAGAATTCTTTTTCAATCTGATGGA16680.10005950781279994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)