FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B04_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B04_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988686
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC85760.28694884641611734No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA47260.15812969311597136No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT46580.15585444573300775No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG43090.14417707313515035No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGATGCATTTGAAAAAAAGGCG40030.13393845991181408No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA39110.13086018404074568No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA36460.12199341115125509No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT36170.12102308506146178No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA36060.12065503033774709No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGCTTGTTGGCTT34680.11603761653114444No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTAGTCGTATCAAAA32230.1078400340484079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)