FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479210
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC104210.42033551010200826No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG99840.402708927440596No Hit
GTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88870.3584609613546251No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG66800.26944066860007826No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT63430.25584762888178086No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT62170.25076536477345607No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA61670.2487485933018986No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA55830.2251927025141073No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT55180.22257089960108262No Hit
ACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACT54590.22019110926464477No Hit
GTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCG54410.21946507153488412No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC51050.2059123672460179No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGGGGACTTGAA51000.20571069009886214No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTT48370.19510247215846985No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG47650.19219832123942707No Hit
GTGTTACCATTCCATTCAAGTCCCCCGATGAGGACCCCAACTGCATTTTT46250.18655136111906614No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT45330.1828405016114004No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT44610.17993635069235764No Hit
CATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA40740.1643265395025028No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA38270.1543636884330089No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC37870.15275027125576293No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAA37660.1519032272377088No Hit
ATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGA37600.1516612146611219No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC36320.14649827969393478No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC34980.14109333215016073No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT34250.13814884580168682No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACATTGAT33920.13681777663045888No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACA33380.13463966344117684No Hit
TGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA33100.13351027141710464No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAAT32940.13286490454620625No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC32650.13169517709270293No Hit
GTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC32250.13008175991545695No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT32000.1290733741796782No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA30550.12322473691216153No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC30190.12177266145264015No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGT29220.11786012479781867No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG28800.11616603676171038No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATT28420.11463329044332671No Hit
ATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG28310.11418960071958406No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC28030.11306020869551188No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG27990.11289886697778727No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC27920.11261651897176922No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAAT27900.11253584811290694No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTCGGGTGTC27850.1123341709657512No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC27400.11051907664134945No Hit
TCTCATTTCTGCTCTGGAGGTAATGAAGGTCTCCCATTCTCATCACAGCT27260.10995438062931337No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC26350.10628385655107878No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA26270.10596117311562957No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA26050.10507379366814429No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC26040.10503345823871313No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT25800.10406540793236554No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC25750.1038637307852098No Hit
CCCTTAGTAGTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTC25190.10160494673706544No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC24970.10071756728958015No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)