FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296993
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA48590.37463579217466864No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT47860.3690073886289286No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28650.22089556381568753No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT21720.16746428084037462No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT20950.1615274716208954No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20160.1554364595645466No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT19070.14703240495515396No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA16940.13060980282854265No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTGCAGTAACT16670.12852806453080318No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16230.1251356021196722No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG15690.12097212552419326No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT15250.11757966311306228No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT14470.11156575247514829No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT13800.10639995744001701No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC13640.1051663347450603No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT13450.1037014077947992No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC13370.10308459644732083No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA13230.1020051765892337No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA13220.10192807517079892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)