FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B01_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B01_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431256
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTAGTGATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA31700.22148378766621762No Hit
CTACCTCTGGATTTTTAGTCAAATTTCTTCAAAGCCAGTTTGAAATGAGC30270.21149256317528098No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG26940.18822628516491807No Hit
CACGTGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG24860.17369359499628298No Hit
GTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAGAGATGAAG24260.16950147283225364No Hit
TTATTAAATCATGCCACATTATTTTGTAGTGATGGAGGGCAGTTGAATAA23600.16489013845182135No Hit
ATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAGAGATGAAGGAAAA21370.1493094177421789No Hit
GTAGTAACAAGAGCCAGCACGCTGTGACATATCGATGTTGTGATGAGCTT17960.1254841901099454No Hit
CACGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG17490.12220036108145572No Hit
GTGGAGAGATGAAGGAAAAGAGAGAGCCAAATAGATTATTAAATCATGCC17120.11961521908030429No Hit
GTAGTAACAAGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAA16710.11675060226821755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)