FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_B01_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_B01_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436431
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC78690.3229724133373775No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC73150.30023423606086114No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC59710.2450715821625977No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA58790.2412955671636094No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA47090.19327450685038894No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG46560.19109919386184135No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC42980.17640557027882176No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA39000.16007020104406813No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG38680.15875680452268093No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG37140.1524360837635049No Hit
GAGTTTATAAAATCTGATGGCCACGAATCTAATTTTATGAGACAGTGTAA37090.15223086555703813No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA36570.1500965962097839No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC33750.13852228936505898No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT32220.1322426122471763No Hit
GAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCA31160.1278919862700811No Hit
CTGTAGGGAAATCCAGGAGATAGTTAAACACAGGCAAGGGGCTGGAGCTC31130.12776885534620108No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT30940.12698902616162738No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA30910.12686589523774736No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA29330.12038099991339793No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA29280.12017578170693116No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC28290.11611246121888943No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT28230.11586619937112932No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA27760.11393714823034184No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT26060.1069597292104722No Hit
GGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACAT25760.10572841997167168No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG25490.1046202416567512No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT25360.10408667431993765No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA25040.10277327779855043No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC24550.10076213937517622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)