FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A12_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A12_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071600
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC35330.32969391564016426No Hit
GTGTAATGATAAAAGGAATGAGGGAGGCTGATAAATACAGATCTGTATGT20240.18887644643523704No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA19180.1789846957820082No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA16550.15444195595371407No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC16430.15332213512504667No Hit
GAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCA15550.14511011571481897No Hit
GATAAATACAGATCTGTATGTCTTCCAGTTTTAGCCAGCTTGTCTTGTTT15530.14492347891004106No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG15070.1406308324001493No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA13590.12681970884658456No Hit
GGATAAATGAAGGAACTGAACCAGCTAGGACCAAAATCAGTAATTTGAAC12570.11730123180291153No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC12540.11702127659574468No Hit
GTATTTACCACTCACACATCTGAGGAGAGAAAGTAGGAGGTGCTGGGGGG11720.10936916759985069No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG11600.10824934677118327No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA11350.1059163867114595No Hit
TTATGAGACAGTGTAATGATAAAAGGAATGAGGGAGGCTGATAAATACAG11050.10311683463979097No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)