FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A11_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A11_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116864
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT56640.507134261646897No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA36230.3243904360781617No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT34810.3116762649704888No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT32300.2892026244914332No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG32290.28911308807518193No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT30450.2726383874849579No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC29040.26001375279353617No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG27990.2506124290871583No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC26870.24058435046702195No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC23450.20996289610910548No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG23090.20673958512406168No Hit
CATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA22170.1985022348289496No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT22040.1973382614176838No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT21820.195368460260157No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA20420.18283336198498654No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC19820.17746117700991348No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG19300.17280528336485015No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT19220.1720889920348404No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA19050.1705668729585697No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACA19010.17020872729356484No Hit
ATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGA18880.169044753882299No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAAT18240.1633144232422211No Hit
TGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA17790.15928528451091628No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACATTGAT17240.1543607816170993No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA16940.15167468912956278No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATT15910.14245243825568735No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT15760.14110939201191908No Hit
TCTCATTTCTGCTCTGGAGGTAATGAAGGTCTCCCATTCTCATCACAGCT14830.13278250530055585No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC14690.13152899547303878No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTCGGGTGTC14210.12723124749298034No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG13880.12427654575669016No Hit
GTGTAATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACT13780.12338118159417799No Hit
CGATTGGACCAGGCGGTCATGGAAAAGAACATAGTACTGAAAGCGAACTT13530.12114277118789754No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA13380.11979972494412928No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA13130.11756131453784884No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA12910.11559151338032204No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT12910.11559151338032204No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGA12890.1154124405478196No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT12690.11362171222279525No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC12590.11272634806028309No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT12510.11201005673027334No Hit
ATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG12290.11004025557274655No Hit
GGATACATATGCAGTGGGGTTTTCGGAGACAATCCACGCCCTAATGATAA11940.10690648100395393No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC11910.10663787175520029No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA11830.10592158042519054No Hit
GTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAATTTCTGTCCCA11810.10574250759268809No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA11720.10493667984642714No Hit
CATTATGTATAGGGTATCATGCGAACAATTCAACAGACACTGTAGACACA11610.10395177926766375No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT11540.10332502435390523No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA11350.1016238324451321No Hit
GTCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAA11340.10153429602888085No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATACTTTGGAC11330.10144475961262965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)