FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A06_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A06_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433459
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATATACTGCCTAGTAGACATCCGCACATGGAGGACATGAAGGCATC28600.1995173911496597No Hit
CACCTGAGCCTCCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGTGGCTG27920.194773621010437No Hit
GGTGAGATCAGCCACCAAGGAGGCTCAGGTGAGATCAGCCACCAAGGAGG25090.17503116587220144No Hit
GATCTCACCTGAGCCTCCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGT23380.16310197919856795No Hit
ATATAAAGCACTTACTTCCACAGTGCCTGGCAAGTGTTGGAGATCATTAA23060.16086961678011022No Hit
CCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGTGGCTGATCTCACCTGA19690.13736005006072724No Hit
AAACTAAAGTGCCTTTACCATCTTTCCTCCAATTTTAGGCCTTCTTTGTC19570.1365229141538056No Hit
TTATAGATGAGTTCATATACTGCCTAGTAGACATCCGCACATGGAGGACA17850.12452396615459528No Hit
AGCACGCACTTACCAGGTGGTGATGGAGCTAATGGGAGCCAGGTCAAATG16510.11517594852730353No Hit
GCTCAGGTGAGATCAGCCACCAAGGAGGCTCAGGTGAGATCAGCCACCAA16170.11280406345769219No Hit
CCAATATACATCCTCGGGACAGCCATTATAACCCCCTGATGATGTTGGTA15580.10868814524866074No Hit
AGCACGCACTTAGCCGCAGTCTATCAACTAGCTGAGGCAGAACGTGCCGC15040.10492103366751333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)