FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A06_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A06_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412649
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC73510.5203698866455857No Hit
ATATTATTCCCTGAGGACATGGAAATTAGGGGAAGCAATTTACATTCCCT61760.4371928200140303No Hit
ATATATTATTCCCTGAGGACATGGAAATTAGGGGAAGCAATTTACATTCC35480.2511593467308581No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA30430.21541090532750884No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA25890.1832727025609334No Hit
ACTTGACACTGTCTGAAGACATTTTTGGTTGTCACTATCTGGGGAGAAGG18890.1337204075463898No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA18380.1301101689096159No Hit
GTTCTGGAGCACGGGGCCCAGCACAGGGTGAGAGGCAAGATGCCTGTAGG17600.12458862746513819No Hit
GTATGGGGAGGATCTGAAAATTAAACAAACAAATTTGCAGCAATATGCAC17460.12359758156484733No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC17270.12225259070016686No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA17060.12076602184973055No Hit
TGTCAACACTGTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGA16590.11743893918446834No Hit
GCTAGAAGATAACAGAGCTAGGATTCAAACACAGCTCCAAAGCCCACGAA16140.11425343450496195No Hit
TCTCAAGGTAATATATGTGGACTTCTCTCCTTGTCACCCCAATTTCGATG16050.11361633356906067No Hit
CTATATGATAAACGGTCAACCTTACTAATCTTAAGATTTCTCAGGCTTGA15970.11305002162603733No Hit
GCCTAGAACCAGGAGTTCTAGTCTGATGGACAGCCTTTCATCTGGAAGTC15360.10873189306048424No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG14980.1060419113311233No Hit
CCCTAAGGCCAATACTTGACACTGTCTGAAGACATTTTTGGTTGTCACTA14590.10328114060888444No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT14490.10257325068010524No Hit
GTAGTAAACAGTATCTGCAACACTACCAGATTTGTATGATTACAAGGAGA14270.10101589283679102No Hit
CTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGATTCAAACAC14250.1008743148510352No Hit
CTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC14170.10030800290801183No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)