FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A06_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A06_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340818
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA99560.7425317977533118No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA94660.7059869422994023No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC90380.6740661297804773No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG85740.6394603891057549No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG67340.5022307278094417No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT63250.4717269607060764No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA55140.4112414958629732No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC52460.3912536973698146No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT47530.354485097902922No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA45830.3418062705005452No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC44830.3343481367344412No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC44530.33211069660461No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA41980.31309245550104486No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC41920.31264496747507864No Hit
TGGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG41480.3093633886179929No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT41320.3081700872154162No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG40500.302054417527211No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT40450.3016815108389058No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG40040.29862367599480316No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA39790.2967591425532772No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA39050.29124012356636025No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG38580.28773480069629137No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT36650.2733406025277107No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA35940.26804532755377686No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC35170.2623025645538768No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG35150.26215340187855474No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT34240.2553665001514001No Hit
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT34190.2549935934630949No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT33910.2529053160085858No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG33270.24813211039827926No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA32990.24604383294377016No Hit
CTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT32270.24067397663217527No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC32180.24000274459322593No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA31090.2318733787881726No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT30610.2282934745804427No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC30510.2275476612038323No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT30340.2262797784635946No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC30290.2259068717752894No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA29460.2197166207494231No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT28810.21486883380145552No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA28610.21337720704823473No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT28160.21002104685348796No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA27900.20808193207430092No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT27540.2053970039185035No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG27230.20308498245101125No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA27070.20189168104843463No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT26620.1985355208536878No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA26460.1973422194511112No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG26190.19532852333426312No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA26050.19428438460700856No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT25990.1938368965810423No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGT25570.19070448039927865No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA25260.1883924589317864No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG25100.18719915752920976No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC25100.18719915752920976No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT24410.18205304523059804No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG23930.17847314102286813No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC23710.17683235159432525No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT23660.17645944490602006No Hit
GTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAG23170.1728049593606291No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA23050.17190998330869664No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA22970.1713133326074083No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG22940.1710895885944252No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT22840.1703437752178148No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC22710.1693742178282213No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA22670.16907589247757712No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC22310.1663909643217797No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAG22190.1654959882698472No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA22140.165123081581542No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG21650.16146859603615107No Hit
GCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCACCTGT21630.161319433360829No Hit
CAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCT21300.15885824921801467No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA21270.15863450520503156No Hit
AACTAGCAACGTACTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAA20950.1562479023998783No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT20860.15557667036092893No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC20850.1555020890232679No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG20670.15415962494536917No Hit
GCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACT19920.1485660246207912No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT19640.1464777471662821No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT19360.14438946971177297No Hit
CCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCA19360.14438946971177297No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG19320.14409114436112883No Hit
TTGTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCA19300.1439419816858067No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG18880.14080956550404305No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA18810.14028749614041577No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC18800.14021291480275472No Hit
TCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACCAC18750.13984000811444955No Hit
GCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACC18500.13797547467292354No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG18420.13737882397163523No Hit
GCCTAATTCCTCTGACATTTGTTCATTCACATAATGTTCCAGAAACACTT18180.13558887186777027No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG17770.13253103702366764No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC17720.13215813033536244No Hit
GACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAG17680.13185980498471828No Hit
GGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGC17520.13066650358214163No Hit
ACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGG17480.1303681782314975No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTAC17090.12745950606271694No Hit
GAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTA16920.12619162332247927No Hit
GAGCAAAAGAGCATGAGTGCTGAAGTCTGACAAACCAGGGCTTGAATCTC16900.12604246064715718No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGTGGGGAAAG16870.12581871663417404No Hit
AGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACAT16850.125669553958852No Hit
GTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACTTCAGC16760.12499832191990262No Hit
GTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACCACTTC16740.12484915924458055No Hit
CCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCA16720.12469999656925847No Hit
GACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCTCTTTGG16690.12447625255627534No Hit
GCCTCTTTGGGCATAAAGCCCCGCACCTCACTCAGCTTCATTTGTAAAGT16600.123805020517326No Hit
GAGCAAGGCAGGGGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTG16570.12358127650434288No Hit
CCAGACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAAC16440.12261171911474936No Hit
GAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTCCTGG16400.1223133937641052No Hit
GGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCT16270.12134383637451168No Hit
CTTTTGCTCTACCTGCCTTGGCTGCACAGATGCCTCAGTGATCTTCATAT16100.120075953634274No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG16070.11985220962129088No Hit
GAAAAAAACATCCTGAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTG16030.11955388427064673No Hit
GAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTACATTCTAATGAGAG15950.11895723356935842No Hit
AGCGAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG15920.11873348955637529No Hit
GCACATAAACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAG15800.1178385135044428No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG15780.11768935082912073No Hit
GAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACTTCAGCACTCATGC15780.11768935082912073No Hit
GACACGTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCG15760.11754018815379864No Hit
TGGCTGCACAGATGCCTCAGTGATCTTCATATCTCTGTGGTGTTAAGCAT15720.11724186280315449No Hit
CCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAAGGT15650.11671979343952721No Hit
GGATATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGC15470.1153773293616285No Hit
CAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACA15460.11530274802396746No Hit
ATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAA15400.11485525999800122No Hit
CCTAGTAAATATCCCATGATGGTAATTACAGAAGAGAAGTGGTGGGGAAG15300.11410944662139083No Hit
GCCCCGCACCTCACTCAGCTTCATTTGTAAAGTGAAGATTGTGGTAGTGT15260.11381112127074666No Hit
TCTCAGCTCTGCTCCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCT15190.1132890519071194No Hit
GGCTCATCCTTGTGAATTGGTGCTGGTGATAAACACCTGGTAGTATTCTC15150.11299072655647524No Hit
GGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT15090.11254323853050899No Hit
CTCATAGTCCTAGCTACTTTAGGAGGCTGAGGCAGAAGGATTGCTTAAGC14940.11142451846559338No Hit
GTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCCTATGAATC14930.11134993712793234No Hit
GTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGC14890.11105161177728819No Hit
AGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACG14890.11105161177728819No Hit
GCTCTGCTCCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACC14830.11060412375132195No Hit
ATTCTAATGAGAGGGAGACACGTACTGAACAAGTAATTTCAGTGTGCTAG14710.10970914769938948No Hit
GATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACAC14540.10844126495915181No Hit
GGCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCC14480.10799377693318556No Hit
GCACAGATGCCTCAGTGATCTTCATATCTCTGTGGTGTTAAGCATGAAAC14460.10784461425786349No Hit
GCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGG14380.10724796355657516No Hit
AGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGT14260.1063529875046427No Hit
CTTTCTACCTGAGGTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCA14150.10553259279037126No Hit
ATATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTA14010.1044884540631167No Hit
AGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTT13810.1029968273098959No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTAC13660.1018781072449803No Hit
CTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAGTAAT13660.1018781072449803No Hit
TTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTT13560.1011322938683699No Hit
CACATAATGTTCCAGAAACACTTAAGGGGAGGCAGGCATTAGGCTGAGGG13440.10023731781643742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)