FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_A02_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_A02_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623447
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC57750.3557245786280673No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31560.19440117232037757No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC30410.1873174794126325No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA26390.16255535290034107No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG25620.1578123585186335No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA23720.146108865888446No Hit
GAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCA20850.1284304322838996No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC20230.12461139784668054No Hit
GTGGCAGATGGAATCGGGGAGACTCTGCCAGCAGAACATGTGGGCAAAGC20160.12418021653925258No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG18910.11648055033518187No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC18750.11549499306106083No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT17300.10656338026433879No Hit
GGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACAT17120.1054546283309526No Hit
GGCAAGGGGCTGGAGCTCAGGAGAGGCTTGGTCTGGAAGGAAAAAGTTGA16300.1004036473010822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)