FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n02_lbstarf_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n02_lbstarf_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46223329
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1544540.3341472874011303No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1093070.23647582803912717Illumina Single End PCR Primer 1 (100% over 58bp)
GTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCAC553980.11984857256819385No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT518530.11217928505322496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC269000.049.23549
CGCCGTA270950.048.97949647
GCCGTAT274900.048.25208348
CGTATCA299750.044.265250
GGTCGCC313450.042.46613344
ATCTCGG314200.042.04048536
TCTCGGT317450.041.37156337
CTCGGTG321250.040.87264338
GTCGCCG327850.040.66518445
TGGTCGC326150.040.57819743
TCGGTGG325350.040.56998439
CGGTGGT342700.038.6472940
GTGGTCG352600.037.72933242
GATCTCG351650.037.48435635
AGATCTC382750.034.96064434
TCGCCGT412200.032.02707346
GTATCAT448950.029.4083751
GTAGATC462550.029.1459932
AGTGTAG497700.027.29013829
GTGTAGA503100.027.02446730