FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n02_lbstard_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n02_lbstard_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17661711
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484980.2745940073416443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC57600.028.39028749
GCCGTAT58600.028.37599448
CGCCGTA60400.027.75763947
CGTATCA71650.023.3048150
TCTCGGT76950.021.4237537
ATCTCGG79000.020.6492436
GGTCGCC81050.020.59788744
TCGGTGG82500.020.1918839
TGGTCGC85350.019.68049643
CTCGGTG87650.018.76917338
TCGCCGT89150.018.72783346
GTGGTCG90700.018.55734342
GTCGCCG91950.018.19408245
CGGTGGT90650.018.18663240
GATCTCG90150.018.17154335
AGATCTC105600.015.83941934
GTATCAT119300.014.3434651
CGCTTAG27950.012.96162964
CCTGCGG57100.012.810369561
GCGGCTC55850.012.66466967
Produced by FastQC (version 0.11.2)