FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n02_lbhearta_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n02_lbhearta_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589057
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA1377801.4368461883165362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1152861.2022662916697648No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG763620.7963452506330914Illumina Single End PCR Primer 1 (100% over 58bp)
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA662500.6908917112496046No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA642460.6699928887689374No Hit
ATCCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGT352650.36776296146743104No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC292150.30467020896841057No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG250740.26148556630751074Illumina Single End PCR Primer 1 (100% over 58bp)
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC239630.24989944266678152No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT181210.18897582942723146No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC179480.18717168956238345No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG174330.18180098418436766No Hit
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC173880.18133169924842454No Hit
CTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACGCCGCGATCTCGCCTAAGG167230.17439671075059832No Hit
TCCATGCACCATTATTCAGGCTGAAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCCCGGCCACC163200.1701940034353743No Hit
ACTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTC159550.16638758117716893No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG154420.16103773290741727No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTACCC141560.1476266122935759No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTAC139480.14545747303410544No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT137280.14316319112505016No Hit
GTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCG128970.1344970626413004No Hit
CCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCG103580.10801896369997592No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACC102140.10651725190495792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC153800.061.84263649
GCCGTAT156250.061.20253848
CGCCGTA156250.061.04448347
CGTATCA157600.060.7239650
TGCCTGC218750.060.6238171
GTATCAT173500.054.3037551
GGTCGCC179600.053.44817744
GTCGCCG182400.052.53479445
TGGTCGC186950.051.2160943
CCCGCTA243000.048.9860669
TCCGGAA260600.047.76264648
CTCCGGA261850.047.71634747
AGCCGTT263350.047.67313427
CCGGAAT260800.047.62143749
TAGCCGT264750.047.53828426
TCATTAA201400.047.46795354
GCCTGCT279850.047.4637682
GCTGCCT278350.047.3673136
CTCTCCG266350.047.1139645
GTAGCCG269050.047.0861925
Produced by FastQC (version 0.11.2)