HK3FCAFXX_n02_bay54b_fem.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HK3FCAFXX_n02_bay54b_fem.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16302257
Sequences flagged as poor quality	0
Sequence length	75
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG	335321	2.0568992379398754	Illumina Single End PCR Primer 1 (100% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG	96097	0.5894705254615972	Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	46009	0.28222472507947827	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG	34158	0.2095292694747727	Illumina Single End PCR Primer 1 (100% over 58bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCGTATC	62800	65.33539	49
GCCGTAT	63445	64.94106	48
CGCCGTA	63925	64.50003	47
CGTATCA	64345	64.067055	50
GGTCGCC	66965	61.93047	44
GTCGCCG	67155	61.705784	45
TGGTCGC	67525	61.1802	43
TCGCCGT	68170	60.395306	46
GTGGTCG	69270	59.777496	42
GTATCAT	68620	59.321556	51
CGGTGGT	70715	58.48014	40
TCGGTGG	71265	57.830383	39
TCTCGGT	71580	57.346077	37
CTCGGTG	71560	57.18124	38
TCATTAA	73220	56.17599	54
ATCATTA	73215	56.165867	53
ATCTCGG	73445	56.085903	36
GATCTCG	74735	54.8389	35
GGTGGTC	75520	54.77536	41
CATTAAA	76945	53.963947	55