FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n02_bay54a_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n02_bay54a_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13356862
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2618671.9605428280983963Illumina Single End PCR Primer 1 (100% over 58bp)
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA958630.7177060001069113No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG782830.5860882593531325Illumina Single End PCR Primer 1 (100% over 58bp)
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA530390.3970917720045322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG468590.35082341945286255No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG264580.19808544851328105Illumina Single End PCR Primer 1 (100% over 58bp)
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC258570.19358588866157334No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC201540.15088873419520243No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG167470.12538124598427386No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC165800.12413095231499734No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT161540.12094158044007643No Hit
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC157650.11802921973739042No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA157240.11772226141140037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC481600.065.6299449
CGCCGTA484450.065.4606747
GCCGTAT484050.065.4245748
CGTATCA491000.064.6685750
GGTCGCC502900.063.15560544
TGGTCGC504000.062.84573443
GTCGCCG507300.062.71123545
TCTCGGT511300.061.66097637
CGGTGGT519700.061.0378140
CTCGGTG515150.061.0133738
GTGGTCG520500.060.98512342
TCGGTGG519350.060.8861239
ATCTCGG520900.060.78829636
GTATCAT522150.060.1034651
AGATCTC543450.058.38607834
GATCTCG550900.057.14554235
GGTGGTC559900.056.59547841
TCATTAA573750.055.28856754
GTAGATC580700.055.0280932
TAGATCT582900.054.6477933
Produced by FastQC (version 0.11.2)