FastQCFastQC Report
Wed 1 Mar 2017
HK3FCAFXX_n02_bay46a_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3FCAFXX_n02_bay46a_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20713705
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA2316261.118225831641418No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2265951.0939375645255158Illumina Single End PCR Primer 1 (100% over 58bp)
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA1479180.7141069161697533No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA1321020.6377516721417053No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC905340.43707294276905073No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG694820.33543974870743787Illumina Single End PCR Primer 1 (100% over 58bp)
TCCATGCACCATTATTCAGGCTGAAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCCCGGCCACC685300.3308437577922443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG676040.32637328763733964No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG609560.29427859477577767No Hit
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC559350.2700386048753711No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC559310.2700192939891729No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG400590.19339369755434868No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT385120.1859252123171591No Hit
GTCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCAT381560.18420654344551107No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACC313270.15123803298347638No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC309070.1492103899326557No Hit
ATCCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGT308660.14901245334912322No Hit
GTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCG284990.13758523644128368No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTC258960.1250186772477449No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG251870.12159582266909759No Hit
TTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCC249190.120301993293812No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG228690.11040516411718715Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT226550.1093720317055785No Hit
ACTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTC225480.10885546549977418No Hit
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCC220410.10640781067414062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT444300.063.41208648
CCGTATC443100.063.3522449
CGCCGTA445400.063.29934747
CGTATCA451600.062.44293650
GGTCGCC472500.059.86062244
TGGTCGC478500.058.98476443
GTCGCCG484050.058.49796745
TCTCGGT480800.058.30396337
ATCTCGG487050.057.83847836
GTATCAT492600.056.78319551
CTCGGTG496450.056.42595338
TCGGTGG505900.055.7546639
GTGGTCG508000.055.62017442
CGGTGGT509200.055.53611440
AGATCTC517850.054.5897334
TGCCTGC266750.054.3938751
GATCTCG539200.052.0513535
GTAGATC555600.051.2050432
TAGATCT560750.050.7577733
GGTGGTC557750.050.7396241
Produced by FastQC (version 0.11.2)